Instruments

The FCV Core Facility currently houses the following instruments: BD LSRFortessa SORP, Beckman Coulter Cyan ADP 9, Beckman Coulter MoFlo Astrios, and an AutoMACS Pro Magnetic Cell Separator.

BD LSRFortessa – Analyzer (submicron particle interrogation capabilities)

BD LSRFortessa – Analyzer

Lasers:

200mW – 488nm (Blue) solid state
50mW - 561nm (Yellow-Green) solid state
40mW – 640nm (Red) solid state
50mW – 405nm (Violet) solid state

Sample Loading Options:

  • High-Throughput Sampler for 96-well and 384-well plates
  • 12x75mm 5ml polystyrene tubes (Falcon 352052)

BD LSRFortessa - HTS

Lasers & Filter Sets:

405nm 488nm 561nm 640nm
450/50:
Pacific Blue, CFP, BFP, BV421,V450, Hoechst		 530/30:
FITC, GFP, YFP, ZsGreen, CFSE, Alexa 488, BB515		    
525/50:
BV510, V500 Pacific Orange, Cascade Yellow		   586/15:
PE, Ds-Red, TRITC		  
610/20:
BV605		   610/20:
PE-Texas Red, PI, mCherry, mStrawberry		  
670/30:
BV650		   670/30:
PE-Cy5, 7AAD		 670/30:
APC, Alexa 647, Cy5, DRAQ5
710/50:
BV711		 710/50:
PerCP, PerCP-Cy5.5		 710/50:
PE-Cy5.5		 730/45:
Alexa 700
780/60:
BV786		   780/60:
PE-Cy7		 780/60:
Alexa 750, APC-Cy7, APC-H7

Cyan ADP 9 – Analyzer

Cyan ADP 9 – Analyzer

Lasers & Filter Sets:

488nm solid state laser
405nm solid state laser
642nm diode laser

Lasers & Filter Sets:

Examples of fluorochromes that can be used:

Lasers & Filter Sets: Examples of fluorochromes that can be used

Sample Loading Options:

  • 12 x 75mm tubes
  • 96-well plates (U-bottom, V-bottom, flat bottom) and 384-well plates with HyperCyt® Autosampler

The HyperCyt® Autosampler is a plate reader capable of running samples in 96 and 384-well format.  Please contact the Facility Manager for instructions on the use of the plate reader. 

MoFlo Astrios – Sorter

MoFlo Astrios – Sorter

Lasers & Filter Sets:

Examples of fluorochromes that can be used

405nm 488nm 532nm 561nm 592nm 642nm
448/59:
Pacific Blue,
BV421,V450 		513/26:
FITC, eGFP 		       
546/20:
BV510,
Pacific Orange, Cascade Yellow 		576/21:
PE 		576/21:
PE 		579/16:
PE,
Ds-Red 		   
614/20:
BV605 		620/29:
ECD,
PE-TexasRed 		622/22:
PE-Alexa 610, Propidium Iodide 		614/20:
mCherry
mStrawberry 		620/29
Texas Red
mCherry
mKate
mRaspberry 		 
795/70:
BV785 		664/22:
PE-Cy5,
PerCP 		664/22:
PE-Alexa647,
7AAD 		  671/30
mPlum
 671/30:
APC,
Alexa 647
  710/45:
PE-Cy5.5 		692/18:
PE-Cy5.5 		692/75:
PE-Cy5.5 		722/44 722/44:
Alexa 700
  795/70:
PE-Cy7 		736/47:
PE-Alexa 700 		  795/70 795/70:
Alexa 750,
APC-Cy7

Sample Loading Options:

  • 12 x 75-mm polypropylene tubes
  • 1.5ml eppendorf tubes
  • 15ml tubes
  • 50ml tubes

Collection devices

  • Up to 6-way sorting into 12 x 75 mm polypropylene tubes
  • 4-way sorting: up to three 12x75mm polypropylene tubes and one 15 mL tube
  • 2-way sorting into 50ml tubes
  • One-way sorting (including single cell) onto slides and into 6, 24, 48, 96, and 384-well plates

Nozzles (nozzle should be 4-5 times the cell size)

  • 70 and 100um

AutoMACS Pro – Magnetic Cell Separator

AutoMACS Pro – Magnetic Cell Separator

The Miltenyi Biotec AutoMACS Pro is an automated bench-top magnetic-bead based cell separator designed to be used with MACS reagents to perform positive selection, enrichment, or depletion of virtually any cell type.  For kits and further information please refer to the Miltenyi Biotec website

How to Use:

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