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1. Zeiss LSM 510 META NLO Two-Photon Laser Scanning Confocal Microscope System


Coupled with:
  • Zeiss Inverted Axiovert 200 M Microscope
  • Zeiss CTI Controller 3700
  • Zeiss Tempcontrol 37-2 Digital
 (1)  Laser Lines
  • Argon: 458/477/488/514 nm, 25mW
  • HeNe1: 543 nm, 1mW
  • HeNe2: 633 nm, 5mW
  • Chameleon XR Laser System: tunable, 705 nm – 980 nm


Chameleon laser excites fluorophores from Blue to Red range, but most efficient with UV and near-UV excitable dyes.

(2) Objectives

Objectives available for Zeiss Axiovert 200 M inverted microscope:

Mag.

Objective

NA

Medium

WD(mm)

For 2-Photon

10x

Plan-Apochromat

0.45

Air

2.10

No

20x

Plan-Apochromat

0.75

Air

0.61

Yes

40x

Plan-Neofluar

1.30

Oil (DIC)

0.17

Yes

63x

C-Apochromat

1.20

Water (Corr.)

0.24

No

100x

Alpha Plan-Fluar

1.45

Oil

0.10

No

(3) Fluorescence Filter Sets

Fluorescence filter sets available for use with the Zeiss Axiovert 200 M inverted microscope:

  • CFP filter set
  • FITC filter set
  • Rhodamine filter set
  • YFP filter set
  • DAPI filter set
  • DAPI/ FITC/Texas Red filter set

2. Olympus BX61WI InVivo Two-Photon Confocal Microscope

(1)  Laser Line

  • Coherent Chameleon XR Laser System: tunable, 705 nm – 980 nm

(2) Objectives

Objectives available for Olympus BX61WI upright microscope:

Mag.

Objective

NA

Medium

WD(mm)

For 2-Photon

10x

UMPLFL

0.30

Water

3.30

No

60x

LUMPLFL (IR2)

0.90

Water

2.00

Yes

(3) Filter Sets

 Fluorescence filter sets available for Two-Photon imaging:

  • GFP/DsRed Filter Set (Dichroic mirror: T560LPXR):

Channel 1: GFP (ET510 - 80m 2p) –Emission filter: 470-550 nm

Channel 2: dsRed (ET620 - 60m 2p) –Emission filter: 590-650 nm

 

  • CFP/YFP Filter Set (Dichroic mirror: T505LP):

Channel 1: CFP (ET480 - 40m 2p) –Emission filter: 460-500 nm

Channel 2: YFP (ET535 - 30m 2p) –Emission filter: 520-550 nm

 
3. Image Processing Software
  • Zeiss LSM
  • AutoDeblur – AutoQuant Imaging Inc
4. Preparative Equipments

(1) Cell Culture Room

  • Class II Biological Safe Cabinet, Baker Company
  • CO2 Incubator, SANYO
  • 4 Co Refrigerator, SANYO